405 Touch 마이크로플레이트 워셔 (405 Touch Microplate Washer)
The new 405™ Touch Microplate Washer incorporates a state-of-the-art, high resolution LED backlit touch screen user interface for intuitive and flexible onboard instrument control of 96- and 384-well microplate washing. A 5.7 inch graphical color display seamlessly guides users through protocol development, instrument maintenance and daily use at the touch of a finger. Two USB flash drive ports enable convenient file transfer, storage and operation. For those who prefer computer control or require 21 CFR Part 11 compliance, the 405 Touch can be controlled with optional Liquid Handling Control™ Software.
For over 27 years, BioTek has continued to advance the art of automated microplate washing, resulting in the industry leading ELx405™, which set the standard in full 96- and 384-well washing. The 405 Touch, maintains all the features of the ELx405™ Microplate Washer while adding a new, modern and powerful interface to again reshape the day-to-day routines of today’s life science laboratories. Fast and efficient vacuum filtration, high strength biomagnetic separation, patented Dual-Action™ manifolds, optimized cell washing for loosely adherent cell monolayers and patent-pending Ultrasonic Advantage™ are all available. New key features include automated internal 4-buffer switching and quick release manifolds for easy maintenance and modular use.
Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. BioTek’s washers with their built-in ultrasonic cleaners are the only instruments of their kind available today able to automatically prime with cleaning solution, sonicate manifold tubes to remove protein or salt crystal build up and re-prime with buffer leaving the instrument clean and ready for its next wash. Cleaning these tubes is a time consuming and labor intensive process. BioTek's Ultrasonic Advantage™ incorporates the power of ultrasonic cleaning to make the 405 Touch a self-maintaining microplate washer.
For washing microsphere based assays, all 405 Touch models can be equipped with accessory modules for both vacuum filtration and biomagnetic separation. This allows for full plate washing of polystyrene and magnetic beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The 405 Touch’s vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. Developed with leaders in genotyping, gene expression and protein assays, the 405 Touch’s biomagnetic separation incorporates a range of immobilization options each with high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention. The 405 Touch is Luminex® xMAP® approved.
The 405 Touch Washer, also known as the 405 TS Series, is available in a range of configurations for different microplate formats and wash requirements. The 405 HT TS is designed specifically to rapidly wash 384-well microplates and includes BioTek's patented Dual-Action™ 192-tube manifold to ensure optimal performance. A proprietary tube-in-tube aspiration manifold design also provides complete 384-well evacuation even with buffers not containing surfactant. The 405 Select TS offers the patented Dual-Action™ manifold in a 96-tube format, providing independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection. This unique design washes both 96- and 384-well plates without the need for any hardware changes. With its angled dispense tubes, full control of dispense and aspiration rates and unique X, Y, Z positioning, the 405 Select TS is ideal for the most demanding wash requirements including loosely-adherent cell lines. For those applications that use only 96-well microplates, the standard 405 TS is an ideal choice with unique features to lower background absorbance values and increase the signal-to-noise ratio of typical ELISA assays.
With its color graphical user interface and wide application range, the new 405 Touch Microplate Washer is an easy to use, total solution for 96- and 384-well plate washing in a compact and robot-compatible design.
Applications:
- ELISAs
- MSD assays
- HCS immune cytochemistry
- FLIPR® Ca2+ flux
- Cell-based assays
- Magnetic and polystyrene bead processing (gene expression assays, cytokine assays)
- ELISPOT assays
US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).
xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®
Hardware:
- Accommodates 96- and 384-well microplates
- High resolution, LED backlit touch screen UI for intuitive and flexible onboard washer control
- 5.7 inch graphical color display seamlessly guides users through protocol creation, instrument maintenance and daily use at the touch of a finger
- Fast Intel Atom processor running Windows CE
- Resistive touch screen display
- Supports all gloved environments
- Compatible with widest range in humidity levels
- May be operated with pointing devices
- Low power, green technology - Two USB flash drive ports enable convenient file transfer, storage and operation
- Especially helpful when instrument is shared between researchers
- Insert memory stick, wash plates…and go
- Each unit ships with memory stick containing sample protocols and instructional videos
- 405 HT TS
- Offers fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection
- 405 Select TS
- Accommodates 96-/384-well washing with patented 96-tube Dual-Action™ manifold
- Includes optimized cell washing with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers - 405 TS models automate basic 96-well dispensing and aspiration
- Includes patent-pending Ultrasonic Advantage™ – built in ultrasonic cleaner for manifold self-maintenance
- Equipped with superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
- Also calibrated for accessory 96-tube Dual-Action manifold for 96-well washing
- Automated internal 4-buffer switching
- Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
- Inline filtration within each dispense bottle
- Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
- All washers vacuum filtration ready (accessory filtration modules sold separately)
- All washers biomagnetic separation ready (accessory magnets sold separately)
- Quick change manifold designs
- Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
- BioStack™ Microplate Stacker compatibility for batch processing of up to 50 ANSI/SBS standard height plates
Software:
- Context sensitive help system complete with recommendations, diagrams and images is only a key press away when questions arise
- Instructional videos walk users through instrument setup and basic use
- Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
- Predefined protocols included for out-of-box washing
- ELISA
- Cell washing
- Vacuum filtration and biomagnetic separation – Luminex® xMAP® technology workflows - Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays
- Dispense height
- Flow rate
- Aspiration height
- Soak time
- Shake time and frequency
- Aspiration rate
- Horizontal dispense and aspirate positions
- Crosswise aspiration - AutoPrime
- Additional software flexibility and 21 CFR Part 11 compliance with Liquid Handling Control™(LHC) Software for method development, downloading and washing plates.
- Run protocols created onboard, downloaded from LHC™ Software or transferred on USB drive
Model |
Part # |
96-/384-well |
384-well |
96-well |
Optimized Cell Washing |
4-Buffer Switching |
Ultrasonic Advantage |
Biomagnetic Separation* |
Vacuum Filtration* |
| 405™ HT TS | 405TSHT |
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405TSHTV |
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| 405™ Select TS | 405TSU |
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405TSUV |
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405TSUS |
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405TSUVS |
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| 405™ TS | 405TSR |
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405TSRV |
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405TSRS |
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405TSRVS |
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* 악세사리 부품 별도 판매 가능
| 405™ Touch Microplate Washer 구성 |
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| Feature | Design | |
| HT | Faster 384-well microplate washing |
|
| V | Automated Internal 4-Buffer Switching | |
| S | Ultrasonic Advantage™ |
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| U | 96-/384-well microplate washing |
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| R | 96-well microplate washing |
|
| 개요 | |
| 분석 | ELISA Cell-based assays (model dependent) Magnetic bead, polystyrene bead
Filtration-to-waste processes |
| 분리 | 생체자기 분리(Biomagnetic separation), 진공여과(vacuum filtration) |
| 마이크로플레이트 유형 | 96- , 384-well (모델에 따라) 낮은 프로파일과 표준 높이 솔리드(Solid)와 필터 바닥
|
| 진탕(Shaking) | 60분 까지, 분과 초 단위로 프로그램 작동 가능 강도 - 느린, 중간, 빠른 또는 변경가능 |
| 침액 시간 | 60분 까지, 분과 초 단위로 프로그램 작동 가능 |
| 자석 | 96- , 384-well 높은 견고성의 디자인 BioTek 마이크로플레이트 워셔의 두가지 디자인 중 선택 가능: Flat: - Flat-bottom well - beads pulled to band across well bottom - Round-bottom well - beads pulled to button at well bottom Ring: - beads pulled to 4-zone ring at well bottom |
| 안전/편의성/유지보수 | 폐기물 및 진공 센서, 유체 및 유량 감지 에어로졸(Aerosol) 커버 플레이트 위치에 대한 조정 유틸리티 넘침 방지 프로그램된 관리 루틴 |
| 자동화 | BioStack™ 마이크로플레이트 스태커 - ANSI/SBS 표준 높이 플레이트 50개 까지 (옵션)
|
| 온보드 소프트웨어 | High resolution backlit LED touch screen UI 5.7 inch graphical color display Intel Atom processor running Windows CE Predefined sample wash protocols - ELISA, cell wash, biomagnetic separation and vacuum filtration Quick Menu Create or edit multiple custom protocols Run protocols created onboard, downloaded from LHC™ Software or transferred on USB flash drive |
| PC 소프트웨어 | Liquid Handling Control™ (LHC™), PC를 위한 세척 프로토콜 프로그래밍 및 실행 (옵션) |
| 작동 온도 | 15° C to 30° C (59° F to 86° F) |
| 인터페이스 | PC 제어와 BioStack™ 통합을 위한 – 1 USB port, 1 9-pin RS232 port 파일 전송을 위한 – 2 USB Flash Memory ports |
| 습도 | 10% ~ 85%, 불응축식(non-condensing) |
| 전기 | 100 – 240 Volts AC 50/60 Hz, < 8.0 A |
| 워싱 | |
| 매니폴드 타입 | 96-well washing: 96-tube (8 X 12) manifold 96-/384-well washing: Dual-Action™ 96-tube (8 X 12) manifold 384-well washing: Dual-Action™ 192-tube (16 x 12) manifold |
| 워싱 속도 | Solid bottom plates: 96-Well: 3 asp. / disp. cycles, 300 µL/well, 96-tube manifold, final aspirate: <30 seconds 384-Well: 3 asp. / disp. cycles, 100 µL/well, 96-tube manifold, final aspirate: <80 seconds 1 asp. / disp. cycle, 400 µL/well, 384 wells, 192-tube manifold, final aspirate: <20 seconds Filter bottom plates: 세척 변수 기반에 따라, 가변적임 |
| 유체 전달 | 하나의 내부 양성 이동 펌프 |
| 진공 여과 | 선택적인 진공 단계 (30초 때의 근사값): 가장 낮음: -38 mmHg 낮음: -113 mmHg 보통: -200 mmHg 높음: -390 mmHg 가장 높음: -506 mmHg 진공 여과 시간 범위: 5 ~ 999 초 |
| 용량 범위 | 25 ~ 3,000 µL/well 1 µL 단위로 선택 가능 |
| 버퍼 선택 | 최대 4개의 세척 완충제(buffer)를 위한 내부 자동 전환 (옵션) |
| 흐름 속도 | 1 ~ 11 낮은 흐름, 세포 세척 속도 포함 (모델에 따라 다름) |
| 워싱 주기 | 1 ~ 250 |
| 분주 정밀성 |
|
| 잔여 용량 | Solid bottom plates:
optimized prior to testing
Filter bottom plates:
0.45 µm plate, filtration 30 seconds, low vacuum, blotted
µm plate, filtration 10 seconds, low vacuum, blotted |
| 살균 | 화학물질 0.5% sodium hypochlorite, 70% isopropyl alcohol or 70% ethanol solution |
| 초음파 세척 | 단백질과 소금 결정체 축적을 위해 초음파 용기가 빌트인 되어있습니다. 최대 60분까지, 시간 혹은 분단위로 프로그램이 가능합니다. (모델에 따라 다름) |
| 공급 용기 용량 | 4 L 또는 10 L (옵션) |
| 물리적 특성 | |
| 치수 | 깊이(D): 17" (43.2 cm) 폭 (W): 14" (35.6 cm) 높이(H): 10" (25.4 cm) |
| 무게 | 내부 완충제(buffer) 전환 포함 - 36 lbs (16.5 kg) 비포함 - 30 lbs (13.5 kg) |
| 규제 | |
| 규제 | 체외 진단 사용을 위하여, 모든 BioTek 마이크로플레이트 장비는 CE 와 TUV 마크가 표시되어있습니다. |
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옵션

BioStack 마이크로플레이트 스태커

Liquid Handling Control 소프트웨어
표준(포함) | ||
장비 사용설명서 | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |





