Activating Lasting Change in Childhood Cancer Treatments
Children’s Cancer Hospital Egypt (CCHE) 57357
In 2007, the Children’s Cancer Hospital Egypt (CCHE) 57357 was established to create a better tomorrow for children facing a devastating cancer diagnosis. As the largest pediatric oncology hospital in the world, the center offers best-in-class treatment therapies along with comprehensive family-centered quality care. In addition to clinical and wellness services, CCHE is also home to a wealth of cutting-edge researchers focused on improving clinical outcomes and reducing adverse therapeutic events.
At CCHE, Dr. Sameh Ali is Head of the Tumor Biology Research Program and concentrates his team’s efforts on mitochondrial physiology and pathophysiology as well as multi-faceted characterizations of cellular function and death mechanisms. With a broad and detailed research scope, Dr. Ali needed powerful, highly sensitive instrumentation that was also versatile. He evaluated several options and ultimately selected the Cytation™ 5 Cell Imaging Multi-Mode Reader. “We looked at all the application notes and publications using Cytation 5 and determined that it was best suited to fulfill our research and productivity needs,” he notes.
BioTek’s local support staff helped Dr. Ali to configure a Cytation 5 specific to the lab’s needs, and he credits them for providing timely and thorough responses to help guide his decision-making. In fact, he remains in touch with BioTek’s experts for technical support and as additional team members receive training.
The compact Cytation 5 is used for most of the lab’s imaging applications such as measuring viability and cell death response following compound treatment, monitoring mitochondria and mitochondrial membrane potential, and determining calcium dynamics and reactive oxygen species (ROS). Accessories like atmospheric control and reagent injectors ensure that live cell and tissue slice experiments are time- and cost-efficient.
In Dr. Ali’s lab, Cytation 5’s multi-mode detection is used for optical enzyme activity assays, ELISAs, ROS release, calcium sequestration and release, and much more. Imaging and detection are both controlled and automated via Gen5™ Software, which is also used for data and image analysis. Dr. Ali comments, “The combination of multi-well imaging and high-content analysis increases productivity and opens the horizon for experiments that would have been impractical to perform using conventional fluorescence microscopes.”
To learn more about Children’s Cancer Hospital Egypt (CCHE) 57357, visit their web site.
Thanks to Dr. Sameh Ali at Children’s Cancer Hospital Egypt (CCHE) 57357 for sharing his BioTek experience.