product design

Gen5 Software Features for Imaging & Microscopy

From whole organism imaging to high magnification subcellular image analysis, Gen5 helps transform raw data and images into meaningful results. Gen5 Software automates image capture from BioTek’s Lionheart Automated Microscope and Cytation Cell Imaging Multi-Mode Reader, and automates image processing and analysis to produce publication-ready images and quantitative data. This process of Augmented Microscopy™ enables simple, straightforward imaging workflows.

Highly visual workflow interface in Gen5
Highly visual workflow interface in Gen5 3.04


Gen5 Software supports applications enabled with BioTek Lionheart Automated Microscopes and Cytation Cell Imaging Multi-Mode Readers. From image capture and quantitative data collection through, processing and analysis to publication-ready images, movies and data, Gen5 provides an easy, seamless workflow in an uncomplicated interface. Key applications are highlighted in these visual abstracts:


Autophagy Analysis Using Object Spot Counting
Autophagy Analysis Using Object Spot Counting

Cell proliferation label-free
Cell Proliferation - Label-free Confluence

Mitochondrial Membrane Potential Analysis Using Object Spot Counting
Calcium Kinetics

3D NK cells
3D Kinetic NK Cell Cytotoxicity Assay

See other Gen5 supported imaging and microscopy applications on our Applications pages.



Automated Image Capture

Fluo3 Video icon
Z-stack  Mini

Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.

Exceptional Image Processing Tools

Images and data

Gen5’s image processing tools provide exceptional processing capability to facilitate the analysis of complex biologies, including 3D samples, large montaged samples and processes including live cell kinetics. From easy brightness/ contrast adjustments to deconvolution and automated movie file creation, image processing in Gen5 enables optimized visualization and analysis for many applications.

Powerful Image Analysis

subpopulation without mask

Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.

Publication-ready Images, Graphs and Data


Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.


Product Reviews

더 알아보기 견적 요청